DNA sequencing

Describe the limitations of traditional methods of Identifying bacterium. traditionalistic methods include judging by phenotypes characteristics. This could be a problem if two bacteria looked similar because they could easily be confused. summarise the goal of each of the six move of the lab. Sample Prep First we necessitate to extract the desoxyribonucleic acid from the bacterial circumvent PC Amplification Create millions of copies of the initial deoxyribonucleic acid PC purification pick up out DNA from primers and enzymes Sequencing Prep serve more PC to make copies of DNA at various lengths DNA SequencingSequence simple machine runs gel electrophoresis to Identify nucleotides and tempt sequence. Sequence Analysis Computer determines wide-eyed DNA sequence which can be run and compared. Explain how fluorescent markers jockstrap determine a nucleotide sequence. all(prenominal) nucleotide is assigned a authoritative color. Conclusion 1 How can scientists site speci fic bacteria when they are amplifying and canvass the same region of DNA In each species? They can identify it because in that respect are unique sequences for each species. If you tackle a judge and find the DNA of it you can then compare it to the Donas of cognize bacteria to discover what bacteria the sample is from